Coding

Part:BBa_K2819008:Design

Designed by: Nanda Wang   Group: iGEM18_NUS_Singapore-A   (2018-09-05)


Coding Region for OsPKS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 87
    Illegal NgoMIV site found at 505
    Illegal NgoMIV site found at 1180
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1147
    Illegal SapI site found at 1035


Design Notes

Silent mutations have been introduced to remove illegal sites, particularly RFC[10] compatibility.

Source

Oryza sativa

References